 |
 |
 |
 |
 |
8 Unit Microfluidic Plate [A8] (8 cell inlets, 2 solution switching) |
||||||||||||||||
The A8 plate is designed for multiplexed time-lapsed flow imaging of adherent and non-adherent cells. Similar to the A2 Microfluidic Plate, but allows simultaneous exposure of 8 cell lines and 8 pairs of flow solutions. Compatible with the ONIX Platform or ONIX micro. |
||||||||||||||||
 |
Ordering A8 Microfluidic Plate
|
|||||||||||||||
Suggested Use: For advanced users who want to perform imaging on 8 separate experiment conditions in a single run. It is recommended that users be familiar with the A2 plate before using the A8 plate. |
||||||||||||||||
Exposure Profile |
|
 |
Binary Switching - Expose cells to time-varying switching between 2 solution inlets. |
Microfluidic Plate Details |
|
|
Figure 1: M8 Well Layout |
The well layout of the A8 device is schematically depicted in figure 1. Each flow unit consists of 5 wells arranged in a single row. There are two flow inlets for solution switching, a culture medium inlet inlet, an open well for imaging, and a flow outlet/cell inlet. The eight units can be operated one at a time, or simultaneously, allowing comparison of up to eight different experiment conditions. |
The microfluidic cell imaging design is depicted in figure 2. Cell culture is facilitated by an innovative plug pump mechanism where a silicone plug is inserted into the cell loading well (similar to a cork) to provide the exact air displacement necessary for cell loading into the imaging area. A perfusion barrier prevents the cells from entering the flow channels, and maintains a favorable environment for cell culture. The loaded plates are then placed in a standard cell culture incubator to allow for cell adhesion and growth with continuous exposure to the solution from the "Medium In" well driven by a passive gravity method. |
|
|
Figure 2: Cell Culture Area |
|
Figure 3: Flow Rates |
Once the cells are ready for flow switching experimentation, the plate is placed on an inverted microscope with the flow control system. This allows pressure driven control of cell exposure to the two inlet solutions (Flow #1 and Flow #2). Due to the small volume of the microfluidics (<100 nl), low flow rates can be used to sustain long term culture. At a pressure of ~2 psi, a single experiment (50 ul) can be operated for up to 16 hours continuous flow. |
Figure 4: Flow Switch Rates |
A #1 thickness glass bottom enables high NA imaging on an inverted microscope. A special glass slide formulated for cell culture promotes adhesion of many cell types without surface coating. The microfluidic plate will fit to any standard 96-well stage holder.
|
|
|
All Material ©2007 CellASIC Corporation Terms of Use | Jobs | Help |
