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M2: Mammalian Cell Microfluidic Plate (2 cell chambers, 2 solution switching) |
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The M2 plate enables time-lapsed imaging of cultured mammalian cells with solution switching. The microfluidic cell culture region ensures optimal cell health during long term microscopy studies. Two independent flow units (with identical flow properties) allow simultaneous imaging of two sets of cell/medium combinations. For use with the ONIX Platform. |
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Suggested Use: Ideal for experiments that require switching between two solutions. |
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Related Products (see all ONIX Products) |
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Time Varying Exposure Between Two Solutions |
Long Term Cell Microscopy |
Switch Profile in Cell Area |
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Microfluidic Plate Details |
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Figure 1 Well Layout |
The M2 microfluidic plate offers the most advanced technology for live imaging of mammalian cells. Our pioneering microfluidic design allows long term culture of cells on your microscope stage while providing real time solution exchange. The easy to use format and fool-proof operation allow any user to run long-term live cell imaging experiments with confidence. The well layout of the M2 plate is schematically depicted in figure 1. Each flow unit consists of 5 wells arranged in a single row. There are two flow inlets for solution switching, a media inlet, an open well for imaging, and a cell inlet/ flow outlet. The two units can be operated simultaneously, allowing comparison of two different experiment conditions with identical flow properties. A #1.5 thickness glass bottom enables high NA imaging on an inverted microscope. The microfluidic plate will fit to any standard 96-well stage holder. The microfluidic cell culture area is depicted in figure 2. The cells are loaded in a large (1mm x 2mm) rectangular culture area surrounded by a perfusion barrier. This barrier localizes cells to the culture area as well as ensures uniform nutrient transport across the chamber. A set of air channels allows gas diffusion into the cell culture area and upstream flow channels (light blue). Due to the high gas permeability of the materials and rapid microscale diffusion, this innovative design maintains a well balanced air supply to cultured cells. Use with the ONIX-TCC to precisely regulate the temperature and CO2 content directly on your microscope stage. A key feature of the M2 plate is the ability to change the solution exposed to the cells in real time. After switching the flow on the control panel, the solution surrounding the cells will completely turn over as reported in Figure 4. Because of the highly laminar flow profile, a sharp boundary interface between the two solutions will "sweep" across the chamber without forming a mixing gradient. Cells closer to the flow inlets will experience proportionately faster exchange rates. The carefully designed microfluidic network enables gentle, yet rapid solution exchange, continuous flows for over 3 days without refilling, and elimination of any cross flows between the inlet solutions. Furthermore, culturing cells in the M2 plate is very easy:
When imaging is not necessary (e.g. during cell attachment or growth), the plate can be kept in a standard cell culture incubator. A built-in gravity perfusion mechanism allows for many days of continuous medium flow even in the absence of a flow controller. The small volume of the microfluidic chamber and design of the perfusion barrier makes this method especially useful for limited cell samples and primary cells. |
Figure 2 Trap Area |
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Figure 3 Flow Rate |
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Figure 4 Solution Switch Times |
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Figure 5 Hela Cell Culture |
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