Why Use ONIX?

Why Use the ONIX?

The ONIX™ is designed to perform any experiment that uses microscopy of live adherent or non-adherent cells. Our method is especially useful for tracking and quantifying fluorescence signals due to the enhanced stability of cells over long periods of time. The ONIX™ system gives you the high quality data you want at an affordable price.

#1	Dynamic Exposure Changes with Adherent and Mammalian Cells

	The ONIX™ was designed to enable live cell media switching experiments. Unlike currently used methods, microfluidics technology allows flow control on the same scale as your cells of interest. The result is the most advanced and easy to use live cell imaging platform on the market.

#2	Imaging Yeast and other Non-Adherent Cells during Media Flow

	The unique design of the Y series plates ensures that non-adherent cells are maintained in a fixed focal plane during the course of the experiment (even during flow). This is achieved by using a microfabricated elastomeric ceiling to physically hold the cells in place. The result is crystal clear images during flow that traditional methods, such as agar pads, cannot offer.

#3	Precise Environment Control During Microscopy

	The cell environment is critical to its behavior. The ONIX microfluidic plates utilize continuous medium perfusion with properties similar to the microvasculature. Unlike dish or slide based methods, our microfluidics promote cell-cell communication, enhance nutrient exchange, use less cells and reagents, and provide improved culture uniformity.

How is this system different than other perfusion devices?
CellASIC's patent-pending microfluidic chamber provides the highest quality cell culture environment on the market. The ONIX plates are the only products that contain micron-scale features proven to enhance cell health and ensure experiment uniformity. Competing methods, such as glass perfusion slides and simple microfluidic dishes provide unreliable cell cultures. In addition, the ONIX system is very easy to use and highly reliable, saving you precious time and money.

What types of experiments can I run with the ONIX?
The ONIX system is particularly useful for time-lapsed live cell microscopy. This covers a large range of possible experiments. Some of the most common uses include: GFP/CFP/YFP expression, protein localization, cell cycle analysis, kinetic response to solution change, and cell motility analysis.

What types of cells can I culture in the micro-chambers?
The chambers were carefully designed to be suitable for even the most sensitive cell types. In general, any cell that can be cultured on a glass slide will thrive in the microfluidic chamber. The small volume of the chamber also makes this system especially useful for primary cell culture.