 |
 |
 |
 |
 |
Y2M: Yeast Cell Microfluidic Plate (2 cell chambers, 2 solution mixing) |
||||||||||||||||||||||
The Y2M plate allows dynamic mixing between the two inlet solutions to expose cells to time-varying concentrations. The optimized cell trapping region ensures a large number of cells and dozens of individual fields of view for cell microscopy. Two completely independent flow units (with identical flow properties) allows simultaneous imaging of two sets of cell/medium combinations. Compatible with the ONIX Platform only. |
||||||||||||||||||||||
 |
Y2M Microfluidic Plates
|
|||||||||||||||||||||
Suggested Use: For experiments requiring a time-varying mixture between two inlet solutions. |
||||||||||||||||||||||
Related Products (see all ONIX Products) |
||
|
|
|
| ---------------------------------------------------------------------------------------------------------------------------------------------------- | ||
Exposure Profile |
|
 |
Dynamic Mixing- Continuous mixing between two inlets to generate time-varying concentrations |
| ---------------------------------------------------------------------------------------------------------------------------------------------------- | |
Microfluidic Plate Details |
|
Figure 1 Well Layout |
The well layout of the Y2M plate is depicted in figure 1. The two flow inlets meet at a microfluidic diffusive mixing element, delivering a fully mixed solution to the cell chambers. The two fully independent units can be operated simultaneously, allowing comparison of two different experiment conditions with identical flow properties. A #1.5 thickness glass bottom enables high NA imaging on an inverted microscope. The microfluidic plate will fit to any standard 96-well stage holder. Validated for S. cerevisiae and S. pombe. |
Figure 2 Trap Area |
The microfluidic cell imaging design is depicted in figure 2. The cell inlet leads to the large (1mm x 3mm) rectangular trapping area. This region consists of three 1mm x 1mm trapping pads of progressively smaller ceiling heights for optimized loading of cells (4-6 micron for haploid, 5-8 for diploid). After loading, cells are held firmly in place by the elastomeric ceiling (see figures 5-7). Nine (3x3) position markers are incorporated into each trap area to facilitate image navigation. Each marker has a missing dot at the location corresponding to the global position in the array and a "I" or "II" mark to indicate unit number. |
Figure 3 Flow Rate |
After the cells are loaded, they can be exposed to mixtures of the two flow inlet solutions. An upstream mixer (not shown) fully combines the two input solutions prior to exposing the cells. By independently varying the volumetric flow rate of the two input streams, a dynamic concentration profile is created. Due to the small volume of the microfluidics (<100 nl), low flow rates can be used to sustain long term culture with rapid exchange rates. |
Figure 4 Solution Switch Times |
After switching the flow on the control panel, the solution surrounding the cells will completely turn over as reported in Figure 4. Cells closer to the flow inlets will experience proportionately faster exchange rates. Time varying profiles can be pre-programmed and executed using the ONIX Flow Control System.
|
Figure 5 S. cerevisiae in the microfluidic trap Courtesy of the Lim Lab, UCSF |
|
Figure 6 S. pombe in the microfluidic trap Courtesy of the Forsburg Lab, USC |
|
|
|
Figure 7 Schematic of cell trapping mechanism |
|
All Material ©2007-2008 CellASIC Corporation Terms of Use | Jobs | Help |
