 |
 |
 |
 |
 |
Y8: Yeast Cell Microfluidic Plate (8 cell chambers, 2 solution switching) |
||||||||||||||||
The Y8 plate is designed for multiplexed time-lapsed flow imaging of non-adherent cells. Similar to the Y2 Microfluidic Plate, but allows simultaneous exposure of 8 cell lines and 8 pairs of flow solutions. Compatible with the ONIX Platform or ONIX micro. |
||||||||||||||||
 |
Y8 Microfluidic Plate
|
|||||||||||||||
Suggested Use: For advanced users who want to perform imaging on 8 separate experiment conditions in a single run. It is recommended that users be familiar with the Y2 plate before using the Y8 plate. |
||||||||||||||||
Related Products (see all ONIX Products) |
||
|
|
 |
| ---------------------------------------------------------------------------------------------------------------------------------------------------- | ||
Exposure Profile |
|
 |
Binary Switching - Expose cells to time-varying switching between 2 solution inlets. |
Microfluidic Plate Details |
|
Figure 1 |
The well layout of the Y8 device is depicted in figure 1. Each unit is similar to those on the Y2 plate, but the wells are divided into 4 quadrants to allow 8 parallel independent units. As in the Y2 plate, each flow unit consists of: two flow inlets (A,B) for solution switching, a cell inlet (C), an open well for imaging, and a flow outlet (E). |
Figure 2 |
Figure 2 shows the trapping site design. Each flow unit contains a 1x1.5 mm trapping site, with three progressive heights to maximize capture of yeast cells. After loading, cells are held firmly in place by the elastomeric ceiling (see figure 7). The two flow inlets enter the cell trapping sites as depicted. The design of the nozzles prevents back flow as well as any cells from blocking the channel. |
Figure 3 |
A key feature of the Y8 design is the ability to change the solution exposed to the cells. After switching the flow on the control panel, the solution surrounding the cells will rapidly and completely refresh according figure 3. Cells closer to the flow inlets will experience proportionately faster exchange rates. |
Figure 4 |
Due to the small volume of the microfluidics (<100 nl), low flow rates can be used to sustain long term culture with rapid exchange rates. A #1.5 glass bottom enables high NA imaging on an inverted microscope. The microfluidic plate will fit to any standard 96-well stage holder. Validated for S. cerevisiae and S. pombe. |
Figure 5 S. cerevisiae in the microfluidic device Courtesy of the Lim Lab, UCSF
|
|
Figure 6 S. pombe in the microfluidic device Courtesy of the Forsburg Lab, USC |
|
 |
|
Figure 7 Schematic of cell trapping mechanism |
|
All Material ©2007-2008 CellASIC Corporation Terms of Use | Jobs | Help |
